Part:BBa_K4190014

Exendin-4 sequence Codon Optimized for S. cerevisiae

Protein coding region for expressing Exendin-4 in S. cerevisiae.

Usage and Biology

Exendin-4 (Ex-4) is a GLP-1 receptor agonist (GLP-1 RA) drug that triggers a cascade response that increases insulin production and sensitivity. It was first found in the saliva of the Gila Monster (Heloderma suspectum) [1]. Ex-4 binds to the human GLP-1 receptor found on pancreatic beta cells and activates adenylyl cyclase which converts ATP into cyclic adenosine monophosphate (cAMP). Increased levels of cAMP causes insulin to be released from pancreatic beta cells [2]. Since Ex-4 is a 39 amino acid naturally occurring peptide, we hypothesize that Ex-4 can be produced in both E. coli and S. cerevisiae as a functional pharmaceutical replacement.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] “Exendin-4: From lizard to laboratory...and beyond,” National Institute on Aging. https://www.nia.nih.gov/news/exendin-4-lizard-laboratory-and-beyond (accessed Sep. 21, 2022).

[2] “Mechanisms of Action and Therapeutic Application of Glucagon-like Peptide-1 - ScienceDirect.” https://www.sciencedirect.com/science/article/pii/S1550413118301797 (accessed Jul. 27, 2022).